The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 10, 2023
Filed:
May. 13, 2019
National University Corporation Hokkaido University, Hokkaido, JP;
Fuso Pharmaceutical Industries, Ltd., Osaka, JP;
Yasuhiko Suzuki, Hokkaido, JP;
Miki Nakagawa, Hokkaido, JP;
Yayoi Kameda, Hokkaido, JP;
Satoru Konnai, Hokkaido, JP;
Tomohiro Okagawa, Hokkaido, JP;
Naoya Maekawa, Hokkaido, JP;
Shinya Goto, Hokkaido, JP;
Yamato Sajiki, Hokkaido, JP;
Kazuhiko Ohashi, Hokkaido, JP;
Shiro Murata, Hokkaido, JP;
Yuzuru Kitahara, Tokyo, JP;
Keiichi Yamamoto, Osaka, JP;
NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY, Hokkaido, JP;
FUSO PHARMACEUTICAL INDUSTRIES, LTD., Osaka, JP;
Abstract
The present invention provides mammalian cell expression vectors that impart to mammalian host cells an ability to produce high levels of foreign gene-derived proteins. A ubiquitously acting chromatin opening element (UCOE) is introduced into an expression vector that has a plasmid DNA integrated into a transcriptional hot spot on the chromosome of a dihydrofolate reductase gene-deficient host cell so that it allows for selection of strains that grow in hypoxanthine-thymidine (hereinafter denoted as HT)-free medium, whereby transformants will produce a protein of interest in increased amounts.