The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 29, 2023

Filed:

Sep. 18, 2018
Applicant:

Lallemand Hungary Liquidity Management Llc, Budapest, HU;

Inventors:

Brooks Henningsen, Salisbury, NH (US);

Sean Covalla, Thetford Center, VT (US);

Rintze Zelle, East Thetford, VT (US);

Allan Froehlich, Norwich, VT (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/81 (2006.01); C07K 14/395 (2006.01); C12N 9/04 (2006.01); C12N 9/10 (2006.01); C12N 9/12 (2006.01); C12N 9/90 (2006.01); C12N 9/92 (2006.01); C12N 9/00 (2006.01); C12P 7/06 (2006.01);
U.S. Cl.
CPC ...
C12N 15/81 (2013.01); C07K 14/395 (2013.01); C12N 9/0006 (2013.01); C12N 9/1022 (2013.01); C12N 9/12 (2013.01); C12N 9/1205 (2013.01); C12N 9/90 (2013.01); C12N 9/92 (2013.01); C12N 9/93 (2013.01); C12P 7/06 (2013.01); C12Y 101/01001 (2013.01); C12Y 202/01002 (2013.01); C12Y 207/01017 (2013.01); C12Y 207/11001 (2013.01); C12Y 501/03001 (2013.01); C12Y 503/01006 (2013.01); C12Y 602/01001 (2013.01);
Abstract

Acetate is a potent microbial inhibitor which can affect the performance of yeast in ethanolic fermentation. The present disclosure provides a recombinant microbial host cell having (i) a first genetic modification for increasing the activity of one or more proteins that function in a first metabolic pathway to convert acetate into an alcohol in the microbial host cell; (ii) a second genetic modification for increasing the activity of one or more proteins that function in a second metabolic pathway to import glycerol in the recombinant microbial host cell (iii) a third genetic modification for increasing the activity of one or more proteins that function in a third metabolic pathway to convert a C5 carbohydrate into ethanol in the microbial host cell. The recombinant microbial host cell comprises and natively expresses native proteins that function in a fourth native metabolic pathway to produce glycerol in the microbial host cell.


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