The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 27, 2023

Filed:

Feb. 13, 2018
Applicant:

The Regents of the University of California, Oakland, CA (US);

Inventors:

Paul S. Weiss, Los Angeles, CA (US);

Steven J. Jonas, Hawthorne, CA (US);

Dan Wilkinson, New York, NY (US);

Adam Z. Stieg, Pasadena, CA (US);

Jason Belling, Los Angeles, CA (US);

Assignee:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
B01L 3/00 (2006.01); H01L 41/08 (2006.01); H01L 41/04 (2006.01); C12N 15/87 (2006.01);
U.S. Cl.
CPC ...
B01L 3/502761 (2013.01); C12N 15/87 (2013.01); H01L 41/04 (2013.01); H01L 41/081 (2013.01); B01L 2400/0436 (2013.01); B01L 2400/0442 (2013.01);
Abstract

A microfluidic-based device and system is disclosed for the high-throughput intracellular delivery of biomolecular cargo to cells (eukaryotic or prokaryotic) or enveloped viruses. Cargo integration occurs due to transient membrane permeabilization by exposure to bulk acoustic waves (BAWs) transduced from surface acoustic waves (SAWs) generated by a rapidly oscillating piezoelectric substrate. In this approach, temporary pores are established across the cellular membrane as cells are partially deformed and squeezed or subject to shearing forces as they travel through the vibrational modes created within the microfludic channel(s) of the device.


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