The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 20, 2023

Filed:

Apr. 21, 2022
Applicant:

Paragon Genomics, Inc., Fremont, CA (US);

Inventors:

Zhitong Liu, Foster City, CA (US);

David Debruyne, Hayward, CA (US);

Jack Dong, San Ramon, CA (US);

Michael Clark, Pleasanton, CA (US);

Yutao Fu, San Marcos, CA (US);

Chenyu Li, Hayward, CA (US);

Vidushi Kapoor, Fremont, CA (US);

Kalyani Patankar, Wheeling, IL (US);

Assignee:

Paragon Genomics, Inc., Fremont, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6874 (2018.01); C12N 15/10 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/6855 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6874 (2013.01); C12N 15/1065 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6855 (2013.01);
Abstract

Methods, apparatuses and compositions for generating highly sensitive and accurate sequencing results of massive parallel sequencing (NGS). The methods and compositions may be referred to as SubDivision-Seq, and may comprise two parts. The first part includes making a target-enriched DNA library, organizing the UMIs on DNA molecules to form primary clones and subdividing the primary clones into subclones. The second part includes sequencing the DNA library by NGS, deducing consensus sequence from each subclone, then deducing consensus sequence in each primary clone.


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