The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 06, 2023

Filed:

Nov. 22, 2019
Applicant:

Shanghai Qizhou Ziyue Biotechnology Co., Ltd, Shanghai, CN;

Inventors:

Zhenhua Tian, Shanghai, CN;

Zhanbing Cheng, Shanghai, CN;

Yanbing Xu, Shanghai, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/02 (2006.01); C12P 9/00 (2006.01); C12N 9/06 (2006.01); C12N 15/00 (2006.01);
U.S. Cl.
CPC ...
C12N 9/0024 (2013.01); C12N 15/00 (2013.01); C12P 9/00 (2013.01); C12Y 104/03003 (2013.01);
Abstract

Provided is a D-amino acid oxidative enzyme mutant. The sequence of the mutant comprises a sequence by mutating the 54amino acid residue N, the 58amino acid residue F, the 211amino acid residue C, and the 213amino acid residue M of the sequence shown in SEQ ID NO:1 or the sequence having at least 76% identity with SEQ ID NO:1. The D-amino acid oxidative enzyme mutant has a higher enzyme activity, enzyme activity stability and/or ammonium resistance than a mild D-amino acid oxidative enzyme mutant. Also provided is an application of the D-amino acid oxidative enzyme mutant in preparing 2-oxo-4-(hydroxymethylphosphinyl)butyric acid.


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