The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 04, 2023

Filed:

Nov. 23, 2015
Applicant:

Battelle Memorial Institute, Richland, WA (US);

Inventors:

Tujin Shi, Richland, WA (US);

Weijun Qian, Richland, WA (US);

Thomas L. Fillmore, Kennewick, WA (US);

Xuefei Sun, Richland, WA (US);

Richard D. Smith, West Richland, WA (US);

Assignee:

Battelle Memorial Institute, Richland, WA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/68 (2006.01); G01N 30/86 (2006.01); G01N 33/50 (2006.01); G01N 30/72 (2006.01); G01N 30/88 (2006.01);
U.S. Cl.
CPC ...
G01N 33/6848 (2013.01); G01N 33/50 (2013.01); G01N 33/6842 (2013.01); G01N 30/72 (2013.01); G01N 30/8631 (2013.01); G01N 2030/8831 (2013.01); G01N 2458/15 (2013.01); G01N 2560/00 (2013.01);
Abstract

Antibody-free processes are disclosed that provide accurate quantification of a wide variety of low-abundance target analytes in complex samples. The processes can employ high-pressure, high-resolution chromatographic separations for analyte enrichment. Intelligent selection of target fractions may be performed via on-line Selected Reaction Monitoring (SRM) or off-line rapid screening of internal standards. Quantification may be performed on individual or multiplexed fractions. Applications include analyses of, e.g., very low abundance proteins or candidate biomarkers in plasma, cell, or tissue samples without the need for affinity-specific reagents.


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