The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 28, 2023

Filed:

Feb. 04, 2015
Applicants:

Beihao Stem Cell and Regenerative Medicine Translational Research Institute, Guangdong, CN;

Peking University, Beijing, CN;

Beijing Vitalstar Biotechnology Co., Ltd., Beijing, CN;

Inventors:

Hongkui Deng, Beijing, CN;

Yuanyuan Du, Beijing, CN;

Yan Shi, Beijing, CN;

Jun Jia, Beijing, CN;

Jinlin Wang, Beijing, CN;

Chengang Xiang, Beijing, CN;

Nan Song, Beijing, CN;

Jun Xu, Beijing, CN;

Ming Yin, Beijing, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/071 (2010.01); A61K 35/407 (2015.01);
U.S. Cl.
CPC ...
C12N 5/067 (2013.01); A61K 35/407 (2013.01); C12N 2501/40 (2013.01); C12N 2501/60 (2013.01); C12N 2501/606 (2013.01); C12N 2506/13 (2013.01); C12N 2506/1307 (2013.01); C12N 2510/00 (2013.01);
Abstract

A method for inducing reprogramming of a cell of a first type which is not a non-hepatocyte (non-hepatocyte cell), into a cell with functional hepatic drug metabolizing and transporting capabilities, is disclosed. The non-hepatocyte is induced to express or overexpress hepatic fate conversion and maturation factors, cultured in somatic cell culture medium, hepatocyte cell culture medium and hepatocyte maturation medium for a sufficient period of time to convert the non-hepatocyte cell into a cell with hepatocyte-like properties. The iHeps induced according to the methods disclosed herein are functional induced hepatocytes (iHeps) in that they express I and II drug-metabolizing enzymes and phase III drug transporters and show superior drug metabolizing activity compared to iHeps obtained by prior art methods. The iHeps thus provide a cell resource for pharmaceutical applications.


Find Patent Forward Citations

Loading…