The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Mar. 21, 2023

Filed:

Apr. 13, 2016
Applicant:

Maxcyte, Inc., Gaithersburg, MD (US);

Inventors:

Linhong Li, North Potomac, MD (US);

Madhusudan Peshwa, Boyds, MD (US);

Assignee:

MaxCyte, Inc., Gaithersburg, MD (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/90 (2006.01); A61K 48/00 (2006.01); C12N 5/0783 (2010.01); A61P 35/00 (2006.01); A61K 35/17 (2015.01); A61K 35/33 (2015.01); C12N 5/077 (2010.01); C12N 9/22 (2006.01); C12N 9/96 (2006.01); C12N 15/11 (2006.01);
U.S. Cl.
CPC ...
C12N 15/907 (2013.01); A61K 35/17 (2013.01); A61K 35/33 (2013.01); A61K 48/00 (2013.01); A61P 35/00 (2018.01); C12N 5/0636 (2013.01); C12N 5/0646 (2013.01); C12N 5/0652 (2013.01); C12N 9/22 (2013.01); C12N 9/96 (2013.01); C12N 15/11 (2013.01); C12N 15/902 (2013.01); C12N 2510/00 (2013.01);
Abstract

Compositions and methods concern the sequence modification of an endogenous genomic DNA region. Certain aspects relate to a method for site-specific sequence modification of a target genomic DNA region in cells comprising: contacting the cells with an activating composition; transfecting the cells with a transfection composition comprising (a) donor DNA and (b) a DNA digesting agent; wherein the donor DNA comprises: (i) a homologous region comprising nucleic acid sequence homologous to the target genomic DNA region; and (ii) a sequence modification region; and wherein the genomic DNA sequence is modified specifically at the target genomic DNA region.


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