The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Dec. 20, 2022

Filed:

Sep. 05, 2018
Applicants:

The University of Tokyo, Bunkyo-ku, JP;

Modalis Therapeutics Corporation, Chuo-ku, JP;

Inventors:

Osamu Nureki, Bunkyo-ku, JP;

Hiroshi Nishimasu, Bunkyo-ku, JP;

Hisato Hirano, Bunkyo-ku, JP;

Shohei Kajimoto, Bunkyo-ku, JP;

Tetsuya Yamagata, Cambridge, MA (US);

Yuanbo Qin, Cambridge, MA (US);

Keith M. Connolly, Cambridge, MA (US);

Iain Thompson, Cambridge, MA (US);

Assignees:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 9/22 (2006.01); A61K 47/54 (2017.01); C12N 15/11 (2006.01); C12N 15/90 (2006.01); C12N 15/113 (2010.01);
U.S. Cl.
CPC ...
C12N 9/22 (2013.01); A61K 47/549 (2017.08); C12N 15/11 (2013.01); C12N 15/902 (2013.01); C12N 15/907 (2013.01); C12N 2310/20 (2017.05); C12N 2310/3513 (2013.01); C12N 2800/80 (2013.01);
Abstract

A mutant SaCas9 protein such as a protein having an amino acid sequence resulting from mutations of glutamic acid at the 782-position to lysine (E782K), leucine at the 800-position to arginine (L800R), asparagine at the 968-position to arginine (N968R), asparagine at the 985-position to alanine (N985A), arginine at the 991-position to alanine (R991A), alanine at the 1021-position to serine (A1021S), threonine at the 927-position to lysine (T927K), lysine at the 929-position to asparagine (K929N), and isoleucine at the 1017-position to phenylalanine (I1017F) in SEQ ID NO: 2 has relaxed restriction on target sequence while maintaining binding ability to guide RNA, and is useful as a tool for gene editing.


Find Patent Forward Citations

Loading…