The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 18, 2022

Filed:

Jun. 12, 2018
Applicant:

Grail, Inc., Menlo Park, CA (US);

Inventors:

Xiao Yang, San Francisco, CA (US);

Hyunsung John Kim, San Francisco, CA (US);

Wenying Pan, Menlo Park, CA (US);

Matthew H. Larson, San Francisco, CA (US);

Eric Michael Scott, Redwood City, CA (US);

Pranav Parmjit Singh, Mountain View, CA (US);

Mohini Jangi Desai, San Mateo, CA (US);

Assignee:

GRAIL, LLC, Menlo Park, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/48 (2006.01); C12Q 1/6874 (2018.01); G16H 50/20 (2018.01); G16H 50/30 (2018.01); G16B 30/00 (2019.01); G16B 5/00 (2019.01); G16B 10/00 (2019.01); G16B 20/00 (2019.01); G16B 15/00 (2019.01); G16B 25/00 (2019.01); G16B 35/00 (2019.01); G16B 40/00 (2019.01); G16B 45/00 (2019.01); G16B 50/00 (2019.01); G16B 30/20 (2019.01); G16B 30/10 (2019.01); G16B 35/10 (2019.01); G16B 35/20 (2019.01);
U.S. Cl.
CPC ...
C12Q 1/6874 (2013.01); G16B 5/00 (2019.02); G16B 10/00 (2019.02); G16B 15/00 (2019.02); G16B 20/00 (2019.02); G16B 25/00 (2019.02); G16B 30/00 (2019.02); G16B 30/10 (2019.02); G16B 30/20 (2019.02); G16B 35/00 (2019.02); G16B 35/10 (2019.02); G16B 35/20 (2019.02); G16B 40/00 (2019.02); G16B 45/00 (2019.02); G16B 50/00 (2019.02); G16H 50/20 (2018.01); G16H 50/30 (2018.01); C12Q 2600/112 (2013.01); C12Q 2600/118 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01); C12Q 2600/16 (2013.01);
Abstract

Cell free nucleic acids from a test sample obtained from an individual are analyzed to identify possible fusion events. Cell free nucleic acids are sequenced and processed to generate fragments. Fragments are decomposed into kmers and the kmers are either analyzed de novo or compared to targeted nucleic acid sequences that are known to be associated with fusion gene pairs of interest. Thus, kmers that may have originated from a fusion event can be identified. These kmers are consolidated to generate gene ranges from various genes that match sequences in the fragment. A candidate fusion event can be called given the spanning of one or more gene ranges across the fragment.


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