The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 30, 2022

Filed:

Mar. 20, 2020
Applicant:

Carl Zeiss Microscopy Gmbh, Jena, DE;

Inventors:

Thomas Kalkbrenner, Jena, DE;

Yauheni Novikau, Apolda, DE;

Martin Beck, Jena, DE;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G06V 10/25 (2022.01); G06V 10/50 (2022.01); G01N 21/64 (2006.01); G02B 21/16 (2006.01); G02B 21/36 (2006.01); G06V 10/98 (2022.01); G06V 20/69 (2022.01);
U.S. Cl.
CPC ...
G01N 21/6458 (2013.01); G02B 21/16 (2013.01); G02B 21/365 (2013.01); G06V 10/25 (2022.01); G06V 10/50 (2022.01); G06V 10/98 (2022.01); G01N 21/6408 (2013.01); G01N 2021/6419 (2013.01); G01N 2021/6421 (2013.01); G06V 20/693 (2022.01);
Abstract

The invention relates to a localization microscopy method for localizing signal sources. Here, at least once for each pixel of a detector, values of an error parameter are ascertained and stored in a calibration data record in a manner assigned to the relevant pixel. Captured image data are used to identify regions of origin of signal sources and fit a point spread function to the pixel values of the respective regions of origin. The respective signal source is localized on the basis of the point spread function. The pixel-specific error parameter of each pixel can be compared to a threshold. If the threshold is exceeded, these pixels are either ignored or replaced by means of interpolation when fitting the point spread function. In addition or as an alternative thereto, the real noise performance of the pixels is ascertained and corrected on the basis of derived pixel-specific error parameters.


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