The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 23, 2022
Filed:
Dec. 02, 2019
California Institute of Technology, Pasadena, CA (US);
Amjad Askary, Pasadena, CA (US);
Michael B. Elowitz, Los Angeles, CA (US);
Mark W. Budde, Arcadia, CA (US);
Carlos Lois, South Pasadena, CA (US);
Luis Sanchez Guardado, Pasadena, CA (US);
Long Cai, Pasadena, CA (US);
James Linton, Pasadena, CA (US);
California Institute of Technology, Pasadena, CA (US);
Abstract
Disclosed herein include systems, methods, compositions, and kits for in situ readout of barcodes, such as DNA barcodes. Barcode constructs containing a promoter (e.g., a phage promoter) that is inactive in live cells can be integrated in the genomes of cells. Cells can be fixed, and phage RNA polymerase can be used for transcription of the barcode to RNA transcripts. The RNA transcripts can be detected using, for example, fluorescent imaging and used to determine barcode sequences.