The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 12, 2022

Filed:

Dec. 14, 2015
Applicant:

The Regents of the University of California, Oakland, CA (US);

Inventors:

Yvonne Y. Chen, Los Angeles, CA (US);

Patrick Ho, Fremont, CA (US);

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/64 (2006.01); A61K 35/17 (2015.01); C12N 15/85 (2006.01); C07K 14/47 (2006.01); A61K 38/48 (2006.01); C07K 16/40 (2006.01); C12N 5/0783 (2010.01);
U.S. Cl.
CPC ...
C12N 9/6467 (2013.01); A61K 35/17 (2013.01); A61K 38/482 (2013.01); C07K 14/47 (2013.01); C07K 14/4702 (2013.01); C07K 16/40 (2013.01); C12N 5/0636 (2013.01); C12N 9/64 (2013.01); C12N 15/85 (2013.01); C12Y 304/21079 (2013.01); C07K 2317/622 (2013.01); C07K 2319/00 (2013.01); C07K 2319/95 (2013.01); C12N 2510/00 (2013.01);
Abstract

Compositions and methods are provided for the cell-mediated targeted killing of diseased cells based on the presence of an intracellular antigen, rather than a surface-bound marker. The targeting cells are modified to express a cytotoxic protein that is delivered into a targeted cell, and after delivery is selectively activated by the presence of a cytoplasmic protein of interest. In one embodiment of the invention, the cytotoxic molecule is a Granzyme B (GrB) polypeptide. In the compositions of the invention, GrB is modified to render its cytotoxic enzymatic functions inactive, until the presence of an intracellular antigen unlocks the GrB molecule to enable enzymatic activities.


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