The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 31, 2022

Filed:

Apr. 30, 2020
Applicant:

Mara Renewables Corporation, Dartmouth, CA;

Inventors:

Zhiyong Sun, Dartmouth, CA;

Michael Milway, Dartmouth, CA;

Kevin Berryman, Dartmouth, CA;

Mercia Valentine, Dartmouth, CA;

Roberto E. Armenta, Dartmouth, CA;

Laura Purdue, Dartmouth, CA;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 7/64 (2022.01); C12Q 1/06 (2006.01); C12P 7/6463 (2022.01); C12P 7/6472 (2022.01); C12N 1/12 (2006.01); C12N 1/14 (2006.01); C12N 1/04 (2006.01); C12P 7/6409 (2022.01); C12P 7/6427 (2022.01);
U.S. Cl.
CPC ...
C12Q 1/06 (2013.01); C12N 1/04 (2013.01); C12N 1/12 (2013.01); C12N 1/14 (2013.01); C12P 7/6409 (2013.01); C12P 7/6427 (2013.01); C12P 7/6463 (2013.01); C12P 7/6472 (2013.01);
Abstract

Provided herein are methods of culturing a microorganism. The methods include providing a container comprising one or more microorganisms in a medium, which has a first carbon to nitrogen ratio; culturing the microorganisms until the culture reaches a threshold indicator; harvesting a portion of the culture while maintaining the majority of the culture in the container; and adding fresh medium comprising a second carbon to nitrogen ratio to the container with the majority of the culture comprising the microorganisms.


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