The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Nov. 30, 2021

Filed:

Feb. 27, 2015
Applicant:

Monsanto Technology Llc, St. Louis, MO (US);

Inventors:

Brent Brower-Toland, St. Louis, MO (US);

Andrei Y. Kouranov, St. Louis, MO (US);

Rosemarie Kuehn, St. Louis, MO (US);

Richard J. Lawrence, Kirkwood, MO (US);

Ervin D. Nagy, St. Louis, MO (US);

Linda Rymarquis, St. Louis, MO (US);

Veena Veena, St. Louis, MO (US);

Assignee:

Monsanto Technology LLC, St. Louis, MO (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/81 (2006.01); C12N 15/82 (2006.01);
U.S. Cl.
CPC ...
C12N 15/8213 (2013.01); C12N 15/8216 (2013.01);
Abstract

The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.


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