The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 23, 2021
Filed:
Jul. 24, 2018
Massachusetts Institute of Technology, Cambridge, MA (US);
President and Fellows of Harvard College, Cambridge, MA (US);
Fei Chen, Cambridge, MA (US);
Andrew C. Payne, Cambridge, MA (US);
Jason D. Buenrostro, Cambridge, MA (US);
Paul Reginato, Cambridge, MA (US);
Edward Stuart Boyden, Chestnut Hill, MA (US);
Shahar Alon, Cambridge, MA (US);
Massachusetts Institute of Technology, Cambridge, MA (US);
President and Fellows of Harvard College, Cambridge, MA (US);
Abstract
The present invention provides methods for analyzing polynucleotides such as genomic DNA. In some embodiments, the disclosure provides a method for preparing and amplifying a genomic DNA library in situ in a fixed biological sample. The method comprises treating a fixed biological sample with an insertional enzyme complex to produce tagged fragments of genomic DNA. The method further comprises circularizing the tagged fragments of genomic DNA. The method further comprises amplifying the tagged fragments of genomic DNA.