The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 21, 2021

Filed:

Dec. 21, 2018
Applicant:

Centrome, Inc., Totowa, NJ (US);

Inventors:
Assignee:

Centrome, Inc., Totowa, NJ (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P 7/26 (2006.01); C12N 9/10 (2006.01); C12N 15/81 (2006.01); C12N 15/52 (2006.01); C12N 9/02 (2006.01); C12N 9/90 (2006.01);
U.S. Cl.
CPC ...
C12P 7/26 (2013.01); C12N 9/0069 (2013.01); C12N 9/1085 (2013.01); C12N 9/90 (2013.01); C12N 15/52 (2013.01); C12N 15/81 (2013.01); C12Y 113/11071 (2015.07); C12Y 205/01029 (2013.01); C12Y 505/01018 (2013.01);
Abstract

This invention provides improved biological synthesis of the apocarotenoid α-ionone in. The final native step involved in the natural apocarotenoid pathway depends on an endogenous farnesyl pyrophosphate synthase (FPPs). From there, heterologous geranylgeranyl pyrophosphate synthase (crtE), phytoene synthase (crtB), phytoene desaturase (crtI), lycopene ε-cyclase (LycE) and a Carotenoid Cleavage Dioxygenase (CCD1) are required to complete the synthesis of α-ionone. Lycopene ε-cyclase from lettuce () or modified cyclase fromwas used to overproduce lycopene which was then cleaved by the carotenoid cleavage dioxygenase from(Ph-CCD1).


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