The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 17, 2021

Filed:

Aug. 11, 2017
Applicant:

Amyris, Inc., Emeryville, CA (US);

Inventors:

Lishan Zhao, Emeryville, CA (US);

Wenzong Li, Emeryville, CA (US);

Gale Wichmann, Berkeley, CA (US);

Aditi Khankhoje, Emeryville, CA (US);

Chantal Garcia De Gonzalo, Richmond, CA (US);

Tina Mahatdejkul-Meadows, Milpitas, CA (US);

Shaina Jackson, Oakland, CA (US);

Michael Leavell, Richmond, CA (US);

Darren Platt, San Francisco, CA (US);

Assignee:

AMYRIS, INC., Emeryville, CA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 9/10 (2006.01); A23L 2/60 (2006.01); C12P 19/56 (2006.01); C12N 15/52 (2006.01); A23L 27/30 (2016.01); C12N 15/81 (2006.01); C12P 19/18 (2006.01);
U.S. Cl.
CPC ...
C12N 9/1051 (2013.01); A23L 2/60 (2013.01); A23L 27/36 (2016.08); C12N 15/52 (2013.01); C12N 15/81 (2013.01); C12P 19/18 (2013.01); C12P 19/56 (2013.01); C12Y 204/01017 (2013.01); A23V 2002/00 (2013.01);
Abstract

Provided herein are compositions and methods for improved production of steviol glycosides in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding aitalica UDP-glycosyltransferase 40087 or its variant UDP-glycosyltransferase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a UDP-glycosyltransferase sr.UGT_9252778, Bd_uGT10850, and/or Ob_UGT91B_1 like. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing steviol glycosides in the host cell. The compositions and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M.


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