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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 11021686 B1

PDFFull Text
Date of Patent:
Jun. 01, 2021

Filed:

Jun. 21, 2016

In vitro production of cholangiocytes

Applicant:

Cambridge Enterprise Limited, Cambridge, GB;

Inventors:

Ludovic Vallier, Cambridge, GB;

Fotios Sampaziotis, Hitchin, GB;

Nicholas Hannan, Wollaton, GB;

Assignee:

CAMBRIDGE ENTERPRISE LIMITED, Cambridge, GB;

Attorneys:

Pamela J. Sherwood

Bozicevic, Field & Francis LLP

Primary Examiner:

Teresa E Knight

Int. Cl.
CPC ...
C12N 5/071 (2010.01); A61K 35/413 (2015.01); G01N 33/50 (2006.01); A61P 1/16 (2006.01);
U.S. Cl.
CPC ...
C12N 5/0672 (2013.01); A61K 35/413 (2013.01); A61P 1/16 (2018.01); C12N 5/067 (2013.01); C12N 5/0676 (2013.01); C12N 5/0679 (2013.01); G01N 33/5008 (2013.01); G01N 33/5044 (2013.01); G01N 33/5091 (2013.01); C12N 2501/11 (2013.01); C12N 2501/115 (2013.01); C12N 2501/119 (2013.01); C12N 2501/15 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2501/36 (2013.01); C12N 2501/385 (2013.01); C12N 2501/415 (2013.01); C12N 2501/42 (2013.01); C12N 2501/999 (2013.01); C12N 2503/02 (2013.01); C12N 2506/14 (2013.01); C12N 2506/22 (2013.01); C12N 2506/45 (2013.01); C12N 2513/00 (2013.01); C12N 2533/90 (2013.01); G01N 2800/08 (2013.01);
Abstract

This invention relates to the efficient generation of cholangiocyte progenitor (CP) cells. Foregut stem cells (FSCs) are cultured in a hepatic induction medium comprising bone morphogenetic protein (BMP) and a TGFβ signalling inhibitor to produce a population of hepatoblasts. The hepatoblasts are then cultured in a biliary induction medium comprising fibroblast growth factor (FGF), retinoic acid and a TGFβ ligand to produce a population of cholangiocyte progenitors (CPs). The cholangiocyte progenitors (CPs) may be matured into cholangiocyte-like cells (CLCs) that display functional properties of Common Bile Duct (CBD) cholangiocytes. Methods, kits, cell populations and uses of these cell populations are provided.

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