The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jan. 12, 2021

Filed:

Jul. 17, 2018
Applicant:

Shanghai Academy of Agricultural Sciences, Shanghai, CN;

Inventors:

Jianyu Liu, Shanghai, CN;

Xiaodong Shang, Shanghai, CN;

Chunyan Song, Shanghai, CN;

Qi Tan, Shanghai, CN;

Zhen Xu, Shanghai, CN;

Qiaozhen Li, Shanghai, CN;

Dan Zhang, Shanghai, CN;

Ruijuan Wang, Shanghai, CN;

Hailong Yu, Shanghai, CN;

Lujun Zhang, Shanghai, CN;

Meiyan Zhang, Shanghai, CN;

Hui Yang, Shanghai, CN;

Yu Li, Shanghai, CN;

Feng Zhou, Shanghai, CN;

Ning Jiang, Shanghai, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/80 (2006.01); C12N 13/00 (2006.01); C12N 15/87 (2006.01); C12N 1/14 (2006.01);
U.S. Cl.
CPC ...
C12N 15/80 (2013.01); C12N 1/14 (2013.01); C12N 13/00 (2013.01); C12N 15/87 (2013.01);
Abstract

A method for genetic transformation of edible mushrooms is provided relating to the technical field of genetic transformation of, enoki mushroom and shiitake. The disclosed method for genetic transformation ofincludes: inoculatingliquid mycelia into a foxtail-millet-grain culture medium, and pre-culturing them at 20-25° C. untilmycelia grow on surfaces of foxtail millet grains. This method uses the foxtail millet grains as an attachment matrix, and during the pre-culturing and co-culturing, the culture substrate is shaken up every day. The method for genetic transformation of enoki mushroom or shiitake includes: inoculation of enoki mushroom mycelia or shiitake mycelia, activated culturing of, andinfecting a foxtail millet grain-enoki mushroom mycelium matrix or a foxtail millet grain-shiitake mycelium matrix.


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