The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 22, 2020

Filed:

Feb. 04, 2016
Applicant:

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, CN;

Inventors:

Chaoguang Tian, Tianjin, CN;

Jingen Li, Tianjin, CN;

Chuannan Long, Tianjin, CN;

Tao Sun, Tianjin, CN;

Liangcai Lin, Tianjin, CN;

Jing Xu, Tianjin, CN;

Qian Liu, Tianjin, CN;

Jingxiao Ji, Tianjin, CN;

Wenliang Sun, Tianjin, CN;

Yanhe Ma, Tianjin, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12P 7/46 (2006.01); C07K 14/37 (2006.01); C12N 15/80 (2006.01); C12N 15/52 (2006.01); C12N 9/00 (2006.01); C12N 9/10 (2006.01); C12P 7/44 (2006.01); C12P 7/50 (2006.01); C12N 9/04 (2006.01); C12R 1/645 (2006.01);
U.S. Cl.
CPC ...
C12P 7/46 (2013.01); C07K 14/37 (2013.01); C12N 9/00 (2013.01); C12N 9/0006 (2013.01); C12N 9/10 (2013.01); C12N 9/1096 (2013.01); C12N 9/93 (2013.01); C12N 15/52 (2013.01); C12N 15/80 (2013.01); C12P 7/44 (2013.01); C12P 7/50 (2013.01); C12Y 101/01037 (2013.01); C12Y 206/01001 (2013.01); C12Y 602/01004 (2013.01); C12Y 604/01001 (2013.01); C12R 1/645 (2013.01);
Abstract

Provided are an engineered strain for synthesizing a dibasic organic acid and preparation and application of same. The engineered strain introduces or up-regulates expression of a positive regulator gene for synthesis of a dibasic organic acid, and/or down-regulates expression of a negative regulator gene for synthesis of a dibasic organic acid, as compared with the origin strain of the engineered strain, the producing capability for producing the dibasic organic acid is improved. The dibasic organic acid comprises malic acid, succinic acid, fumaric acid, oxaloacetic acid, glutaric acid, and adipic acid; the expression product of the positive regulator gene comprises aspartate aminotransferase, glutamic acid-aspartate transporter, C4-dicarboxylic acid transporter, pyruvate carboxylase and malate dehydrogenase, glucose transporter; the expression product of the negative regulatory gene comprises succinyl-CoA synthase, and malic acid-alpha ketoglutarate transporter, and the original strain comprises, and


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