The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 25, 2020
Filed:
Mar. 13, 2014
Applicant:
The Trustees of the University of Pennsylvania, Philadelphia, PA (US);
Inventors:
Arjun Raj, Wynnewood, PA (US);
Marshall Levesque, Rahway, NJ (US);
Assignee:
The Trustees Of The University Of Pennsylvania, Philadelphia, PA (US);
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C07H 21/00 (2006.01); C12Q 1/6886 (2018.01); C12Q 1/6841 (2018.01); C12Q 1/6827 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6886 (2013.01); C12Q 1/6827 (2013.01); C12Q 1/6841 (2013.01); C12Q 2600/156 (2013.01);
Abstract
The invention provides a high efficiency fluorescence in situ hybridization (FISH) method for detecting mutations on individual RNA transcripts, including both exonic and intronic RNA transcripts. In certain embodiments, the method is used to quantify allelic expression at the population and single cell level, and also to distinguish maternal chromosomes from paternal chromosomes in single cells.