The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 18, 2020

Filed:

Jan. 18, 2019
Applicant:

Manus Bio, Inc., Cambridge, MA (US);

Inventors:

Ryan Philippe, Cambridge, MA (US);

Ajikumar Parayil Kumaran, Cambridge, MA (US);

Jason Donald, Cambridge, MA (US);

Krishna Patel, Cambridge, MA (US);

Swati Gupta, Cambridge, MA (US);

Ryan Lim, Cambridge, MA (US);

Liwei Li, Cambridge, MA (US);

Assignee:

MANUS BIO INC., Cambridge, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A23L 2/60 (2006.01); A23L 27/30 (2016.01); C12P 19/56 (2006.01); C12N 9/10 (2006.01); C12N 9/02 (2006.01);
U.S. Cl.
CPC ...
A23L 2/60 (2013.01); A23L 27/36 (2016.08); C12N 9/0073 (2013.01); C12N 9/1048 (2013.01); C12N 9/1051 (2013.01); C12P 19/56 (2013.01); C12Y 114/13079 (2013.01); C12Y 204/01017 (2013.01); A23V 2002/00 (2013.01);
Abstract

The invention provides methods for making steviol glycosides, including RebM and glycosylation products that are minor products in stevia leaves, and provides enzymes, encoding polynucleotides, and host cells for use in these methods. The invention provides engineered enzymes and engineered host cells for producing steviol glycosylation products, such as RebM, at high purity and/or yield. The invention further provides methods of making products containing steviol glycosides, such as RebM, including food products, beverages, oral care products, sweeteners, and flavoring products.


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