The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 07, 2020

Filed:

Apr. 30, 2018
Applicant:

GE Healthcare Bio-sciences Corp., Marlborough, MA (US);

Inventors:

John R. Nelson, Clifton Park, NY (US);

R. Scott Duthie, Schenectady, NY (US);

Rohini Dhulipala, Kendall Park, NJ (US);

Gregory A. Grossman, Halfmoon, NY (US);

Anuradha Sekher, Belle Mead, NJ (US);

Assignee:

GE HEALTHCARE BIO-SCIENCES CORP., Marlborough, MA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/10 (2006.01); C12Q 1/6865 (2018.01);
U.S. Cl.
CPC ...
C12N 15/1096 (2013.01); C12Q 1/6865 (2013.01);
Abstract

Methods of amplification, purification and detection of nucleic acid sequences especially RNA are described. One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded region is complementary to the RNA sequence of interest. The double stranded region may also contain additional functionalities which are then used subsequently in the method.


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