The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 05, 2020
Filed:
Oct. 18, 2017
Drexel University, Philadelphia, PA (US);
The Wistar Institute of Anatomy and Biology, Philadelphia, PA (US);
Temple University—of the Commonwealth System of Higher Education, Philadelphia, PA (US);
Ming Xiao, Huntingdon Valley, PA (US);
Harold C. Riethman, Chesapeake, VA (US);
Wenhui Hu, Cherry Hill, NJ (US);
Jennifer McCaffrey, Collegeville, PA (US);
Drexel University, Philadelphia, PA (US);
The Wistar Institute of Anatomy and Biology, Philadelphia, PA (US);
Temple University, Philadelphia, PA (US);
Abstract
Provided herein are methods of detecting a target nucleic acid sequence. In one embodiment, the method includes contacting genomic DNA with a guide RNA having a portion complementary to the target sequence in the genomic DNA and with Cas9 nickase to produce a single-strand break in the genomic DNA at a specific location adjacent to the target sequence. The method further includes contacting the nicked DNA with a polymerase and fluorescently labeled nucleotide. The fluorescently labeled nucleotide is incorporated into the nicked DNA at the specific location and the target nucleic acid sequence is detected via fluorescent label.