The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 10, 2020
Filed:
Sep. 21, 2015
The Royal Institution for the Advancement of Learning/mcgill University, Montréal, Québec, CA;
Université DE Montréal, Montréal, Québec, CA;
Stéphane Alain Laporte, Outremont, CA;
Yoon Namkung, Pointe-Claire, CA;
Michel Bouvier, Montréal, CA;
Christian Le Gouill, Montréal, CA;
Mireille Hogue, Laval, CA;
Viktoriya Lukasheva, Pointe-Claire, CA;
Hiroyuki Kobayashi, Montréal, CA;
Denis Deblois, Montréal, CA;
Étienne Durette, Saint-Jérôme, CA;
The Royal Institution for the Advancement of Learning/McGill University, Montréal, Québec, CA;
Université de Montréal, Montréal, Québec, CA;
Abstract
Bioluminescence resonance energy transfer (BRET) biosensors for assessing the intracellular localization, internalization and trafficking into cellular compartments of proteins such as receptors, and other biomolecules such as second messengers, are disclosed. These biosensors, which are dependent on the concentration/density of the BRET donor and acceptor in cellular compartments rather that specific protein-protein interactions, use aGFP/Luc BRET pair, which allows the robust and reproducible monitoring of protein trafficking/localization, with a sensitivity compatible with high-throughput screening (HTS). The use of these biosensors for various applications, including assessing/monitoring protein endocytosis, recycling and intracellular trafficking, receptor maturation/rescue by pharmacological chaperones, various endocytosis/exocytosis processes, activation/inhibition, as well as biomolecule concentration/density in different cellular compartments, is also disclosed.