The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 18, 2020

Filed:

Aug. 23, 2018
Applicants:

Massachusetts Institute of Technology, Cambridge, MA (US);

President and Fellows of Harvard College, Cambridge, MA (US);

Inventors:

Edward Stuart Boyden, Chestnut Hill, MA (US);

Fei Chen, Cambridge, MA (US);

Shahar Alon, Cambridge, MA (US);

George Church, Brookline, MA (US);

Paul Warren Tillberg, Cambridge, MA (US);

Adam Henry Marblestone, Arlington, MA (US);

Evan R. Daugharthy, Cambridge, MA (US);

Assignees:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/6874 (2018.01); C12Q 1/6806 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6874 (2013.01); C12Q 1/6806 (2013.01);
Abstract

The invention provides in situ nucleic acid sequencing to be conducted in biological specimens that have been physically expanded. The invention leverages the techniques for expansion microscopy (ExM) to provide new methods for in situ sequencing of nucleic acids as well as new methods for fluorescent in situ sequencing (FISSEQ) in a new process referred to herein as 'expansion sequencing' (ExSEQ).


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