The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 03, 2019
Filed:
Mar. 12, 2018
Cargill, Incorporated, Wayzata, MN (US);
Katrin Grammann, Oer-Erkenschwick, DE;
Jan Wolter, Duesseldorf, DE;
Liv Reinecke, Essen, DE;
Steffen Schaffer, Herten, DE;
Eileen E. Spindler, Lafayette, CO (US);
Wendy K. Ribble, Arvada, CO (US);
Brittany L. Robinson, Wheat Ridge, CO (US);
Catherine B. Poor, Boulder, CO (US);
Tanya Warnecke Lipscomb, Boulder, CO (US);
Hans H. Liao, Superior, CO (US);
David A. Hogsett, Longmount, CO (US);
Ronald J. Evans, Louisville, CO (US);
CARGILL, INCORPORATED, Wayzata, MN (US);
Abstract
A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.