The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 05, 2019
Filed:
Apr. 13, 2012
Luis Felipe Barros Olmedo, Valdivia, CL;
Alejandro San Martin, Valdivia, CL;
Sebastian Ceballo Charpentier, Valdivia, CL;
Wolf B. Frommer, Washington, DC (US);
Luis Felipe Barros Olmedo, Valdivia, CL;
Alejandro San Martin, Valdivia, CL;
Sebastian Ceballo Charpentier, Valdivia, CL;
Wolf B. Frommer, Washington, DC (US);
CENTRO DE ESTUDIOS CIENTIFICOS DE VALDIVIA, Valdivia, CL;
CARNEGIE INSTITUTION OF WASHINGTON, Washington, DC (US);
Abstract
A nanosensor for detecting and quantifying lactate in different types of samples, such as tissues, intra-cellular and subcellular compartments, with high spatial and temporal resolution is disclosed. Methods comprising use of the nanosensor for quantifying the activity of lactate transporters, rates of cellular lactate production and cellular lactate consumption, and rate of mitochondrial pyruvate consumption are also disclosed. Methods for quantifying the transformation in energy metabolism that characterizes cancer cells with single-cell resolution and for detecting interference of candidate drugs with mitochondrial energetics are additionally disclosed.