The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 10, 2019
Filed:
Jun. 18, 2018
Cho Pharma Inc., Taipei, TW;
Academia Sinica, Taipei, TW;
Nan-Horng Lin, Vernon Hills, IL (US);
Lin-Ya Huang, New Taipei, TW;
Sachin S Shivatare, Taipei, TW;
Li-Tzu Chen, Taipei, TW;
Chi-Huey Wong, Taipei, TW;
Chung-Yi Wu, New Taipei, TW;
Ting Cheng, Keelung, TW;
CHO Pharma Inc., Taipei, TW;
Academia Sinica, Taipei, TW;
Abstract
A mutant of EndoS2 includes one or more mutations in the sequence of a wild-type EndoS2 (SEQ ID NO:1), wherein the one or more mutations are in a peptide region located within residues 133-143, residues 177-182, residues 184-189, residues 221-231, and/or residues 227-237, wherein the mutant of EndoS2 has a low hydrolyzing activity and a high tranglycosylation activity, as compared to those of the wild-type EndoS2. A method for preparing an engineered glycoprotein using the mutant of EndoS2 includes coupling an activated oligosaccharide to a glycoprotein acceptor. The activated oligosaccharide is a glycan oxazoline.