The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Aug. 13, 2019
Filed:
May. 27, 2016
Applicant:
The University of Hong Kong, Hong Kong, CN;
Inventors:
Assignee:
The University of Hong Kong, Hong Kong, CN;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); C12Q 1/6827 (2018.01); C12Q 1/6886 (2018.01);
U.S. Cl.
CPC ...
C12Q 1/6827 (2013.01); C12Q 1/6886 (2013.01); C12Q 2600/156 (2013.01);
Abstract
Disclosed are methods of using dsquare planar metal complexes containing tridentate π-conjugated ligands and ancillary ligand of N-heterocyclic carbene or di-phosphine ligand to target mismatched DNA. Targeting of mismatched DNA can be revealed by monitoring the differences in emission enhancement of metal complexes toward mismatched DNA and matched DNA; or the gradually heat release from isothermal titration calorimetry (ITC) when complexes bind toward mismatch DNA; or the significant increase in melting temperature of the mismatched DNA after adding complexes.