The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Aug. 13, 2019

Filed:

Mar. 18, 2016
Applicant:

Kansas State University Research Foundation, Manhattan, KS (US);

Inventors:

Stefan H. Bossmann, Manhattan, KS (US);

Deryl L. Troyer, Manhattan, KS (US);

Aruni P. Malalasekera, Manhattan, KS (US);

Hongwang Wang, Manhattan, KS (US);

Sebastian O. Wendel, Manhattan, KS (US);

Gaohong Zhu, Yunnan, CN;

Assignee:
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
A61K 38/00 (2006.01); A61K 49/00 (2006.01); G01N 33/573 (2006.01); C12Q 1/26 (2006.01); C12Q 1/34 (2006.01);
U.S. Cl.
CPC ...
A61K 49/0093 (2013.01); A61K 49/0032 (2013.01); A61K 49/0036 (2013.01); A61K 49/0056 (2013.01); C12Q 1/26 (2013.01); C12Q 1/34 (2013.01); C12Y 113/11011 (2013.01); C12Y 113/11052 (2013.01); C12Y 305/03001 (2013.01); G01N 33/573 (2013.01);
Abstract

A nanoplatform assembly for detection of arginase, indoleamine 2,3-dioxygenase 1, and/or tryptophan 2,3-dioxygenase. The nanoplatform comprises an oligopeptide, which is used as a linker between two particles. More preferably, the linker is comprised of an oligopeptide containing a substrate for the target enzyme, where the substrate is chemically or physically modified by the target enzyme (but not cleaved). A central particle with a plurality of oligopeptide-tethered detectable particles and a plurality of directly attached detectable particles is described. Posttranslational modification of the oligopeptide leads to changes in the detectable signals from the first and/or second particles in the nanoplatform, which can be correlated to enzyme activity and concentration.


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