The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 12, 2019
Filed:
Dec. 02, 2013
Integrated Dna Technologies, Inc., Coralville, IA (US);
Joseph Alan Walder, Chicago, IL (US);
Mark Aaron Behlke, Coralville, IA (US);
Scott D. Rose, Coralville, IA (US);
Joseph R. Dobosy, Coralville, IA (US);
Susan M. Rupp, Marion, IA (US);
INTEGRATED DNA TECHNOLOGIES, INC., Coralville, IA (US);
Abstract
Methods and compositions are provided for improving specificity during amplification of a target DNA sequence. The methods and compositions rely upon the use of an RNase H enzyme, a polymerase, and RNase H enzyme-sensitive, blocked-cleavable oligonucleotide primers in the amplification reactions, wherein the reaction mixtures include either an optimized final concentration of a divalent metal salt comprising 2.0 mM or less of free Mgcation and/or an optimized final concentration of a non-ionic detergent comprising at least about 0.001% polyethylene glycol hexadecyl ether.