The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 16, 2018
Filed:
Aug. 28, 2017
Zhejiang University of Technology, Hangzhou, CN;
Zhejiang Chiral Medicine Chemicals Co. Ltd., Hangzhou, CN;
Renchao Zheng, Hangzhou, CN;
Yuguo Zheng, Hangzhou, CN;
Qin Zhang, Zhangshi, CN;
Youming Huang, Hangzhou, CN;
Jianfeng Weng, Hangzhou, CN;
Tianchun Liu, Hangzhou, CN;
Weirong Fan, Hangzhou, CN;
ZHEJIANG CHIRAL MEDICINE CHEMICALS CO. LTD., Hangzhou, CN;
ZHEJIANG UNIVERSITY OF TECHNOLOGY, Hangzhou, CN;
Abstract
The disclosure provides a nitrilase from, which belongs to genus, family brassicaceae. The disclosure further provides the encoding gene, vector, recombinant bacterial strain, and the application in the manufacturing of (S)-3-cyano-5-methylhexanoic acid. The wet resting cells containing nitrilase Aa-Nit can kinetically resolve racemic IBSN at 1.2 M with a 42% conversion rate in 15 hr and >99% ee value. The disclosure provides a regio- and stereoselective method for the preparation of (S)-3-cyano-5-methylhexanoic acid. This method provides an atom economical, mild, environmental friendly industrial method to manufacture (S)-3-cyano-5-methylhexanoic acid.