The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Oct. 02, 2018

Filed:

Oct. 30, 2014
Applicant:

Peking University, Beijing, CN;

Inventors:

Demin Zhou, Beijing, CN;

Chuanling Zhang, Beijing, CN;

Sulong Xiao, Beijing, CN;

Tianzhuo Yao, Beijing, CN;

Yongxiang Zheng, Beijing, CN;

Fei Yu, Beijing, CN;

Longlong Si, Beijing, CN;

Lihe Zhang, Beijing, CN;

Assignee:

Peking University, Beijing, CN;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 15/86 (2006.01); C12N 7/00 (2006.01); C07K 14/005 (2006.01); A61K 48/00 (2006.01); A61K 39/00 (2006.01); A61K 39/23 (2006.01);
U.S. Cl.
CPC ...
C07K 14/005 (2013.01); A61K 39/23 (2013.01); C12N 7/00 (2013.01); C12N 15/86 (2013.01); A61K 2039/53 (2013.01); C12N 2750/14121 (2013.01); C12N 2750/14122 (2013.01); C12N 2750/14134 (2013.01); C12N 2750/14143 (2013.01); C12N 2750/14145 (2013.01); C12N 2750/14151 (2013.01);
Abstract

The present invention relates to an adeno-associated virus with site-directed mutagenesis and site-specific modification, and a preparation method and uses thereof. Specifically, the present invention uses genetic code expansion techniques to incorporate non-natural amino acid into an adeno-associated virus capsid protein VP1 or fragment thereof, thereby obtaining an adeno-associated virus with site-directed mutagenesis using the non-natural amino acid. The adeno-associated virus with site-directed mutagenesis is equivalent to a wild-type virus in terms of production, transduction and mobility, can couple with other functional molecules, such as targeting molecules, and can carry a functional gene in a normal manner, which indicates that the adeno-associated virus with site-directed mutagenesis can be used as a tool adeno-associated virus, and applied in various aspects associated with adeno-associated virus such as finding adeno-associated virus binding proteins or using as target genetic therapy vector.


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