The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 19, 2018
Filed:
Oct. 06, 2014
Applicant:
Cambridge Enterprise Limited, Cambridge, GB;
Inventors:
Ludovic Vallier, Cambridge, GB;
Nicholas Hannan, Cambridge, GB;
Assignee:
Cambridge Enterprise Limited, , GB;
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01); G01N 33/50 (2006.01);
U.S. Cl.
CPC ...
C12N 5/068 (2013.01); C12N 5/0672 (2013.01); C12N 5/0678 (2013.01); C12N 5/0689 (2013.01); G01N 33/5023 (2013.01); C12N 2500/92 (2013.01); C12N 2501/11 (2013.01); C12N 2501/115 (2013.01); C12N 2501/119 (2013.01); C12N 2501/12 (2013.01); C12N 2501/155 (2013.01); C12N 2501/16 (2013.01); C12N 2501/385 (2013.01); C12N 2501/40 (2013.01); C12N 2501/415 (2013.01); C12N 2501/91 (2013.01); C12N 2501/999 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01);
Abstract
This invention relates to the differentiation of pluripotent cells (PSCs) into foregut stem cells (FSCs) using a definitive endoderm induction medium comprising a TGFfi ligand, fibroblast growth factor (FGF), bone morphogenetic protein (BMP) and a PI3K inhibitor to differentiate the pluripotent cells into definitive endoderm cells and a foregut induction medium comprising a TGFβ ligand to differentiate the definitive endoderm cells into foregut stem cells (FSCs). Methods of differentiation, populations of foregut stem cells, culture media and kits are provided.