Company Filing History:
Years Active: 2024-2025
Title: Innovations of Inventor Xiaochen Xie
Introduction
Xiaochen Xie is a prominent inventor based in Wuhan, China. He has made significant contributions to the field of biotechnology, particularly in DNA synthesis and assembly methods. With a total of 2 patents, his work showcases innovative approaches to genetic engineering.
Latest Patents
Xie’s latest patents include an efficient synthesis and assembly method for large fragment DNA based on programmable nuclease Argonaute. This method involves constructing and treating antibiotic resistance gene reconstructed vectors with linearization. It also includes dividing a target DNA into multiple small DNA fragments, synthesizing these fragments, and loading them onto the antibiotic resistance gene reconstructed vectors. The combination of SLIC and resistance gene reconstruction allows for the assembly of 5-6 small fragments in a single reconstruction, enhancing efficiency and saving time. Additionally, mutations caused by PCR are avoided, and the reconstructed large fragment does not require a second sequencing, which reduces both time and costs.
Another notable patent is related to the PfAgo mutant protein, which exhibits mesophilic target nucleic acid cleavage activity. This mutant protein has specific amino acid mutations that enhance its activity within a temperature range of 30-95 degrees Celsius. This advancement effectively expands the application range of prokaryotic Argonaute protein, making it a valuable tool in genetic research.
Career Highlights
Xiaochen Xie is affiliated with Hubei University, where he continues to advance his research in biotechnology. His innovative methods and discoveries have positioned him as a key figure in the field.
Collaborations
Xie collaborates with notable colleagues, including Lixin Ma and Wanping Chen, who contribute to his research endeavors and enhance the impact of his work.
Conclusion
Xiaochen Xie’s contributions to biotechnology through his innovative patents demonstrate his commitment to advancing genetic engineering. His work not only improves efficiency in DNA synthesis but also expands the potential applications of Argonaute proteins.