Vaniambadi S Kalyanaraman

Title: Vaniambadi S Kalyanaraman: Innovator in HIV Research

Introduction

Vaniambadi S Kalyanaraman is a notable inventor based in Germantown, MD (US). He has made significant contributions to the field of HIV research, holding a total of 5 patents. His work focuses on developing innovative solutions for the detection and treatment of HIV-2 infections.

Latest Patents

One of his latest patents involves a human T-cell line infected with the HIV-2.sub.NIHZ virus. This novel cell line secretes functionally intact viral envelope proteins in the extracellular medium. The cells demonstrate robust growth in serum-free medium, allowing for a more than 100-fold concentration of the conditioned medium. This concentration step is crucial, as it is otherwise unachievable under conventional conditions that utilize 10% fetal calf serum. A specific affinity procedure is outlined to purify the gp160 protein from the concentrated conditioned medium of 100-liter cultures. This purification employs a mouse monoclonal antibody targeting the HIV-2 gp41 protein, a component of the gp160 protein. The purified HIV-2 gp160 exhibits high specificity and sensitivity when reacting with antiviral antibodies from HIV-2 infected humans, as demonstrated by various methods including ELISA, western blot analysis, and radioimmuno-precipitation of metabolically labeled gp160. This purified protein serves as a diagnostic tool for detecting HIV-2 infections in humans. Additionally, due to its oligomeric properties and similarity to the virion envelope, the purified gp160 holds potential for developing vaccine strategies against HIV-2.

Career Highlights

Throughout his career, Kalyanaraman has worked with prominent companies such as Akzo Nobel N.V. and Akzo N.V. His expertise in the field has led to significant advancements in HIV research and diagnostics.

Collaborations

Some of his notable coworkers include Mangalasseril G Sarngadharan and Irene LaRue-Kalisz. Their collaborative efforts have contributed to the success of various research projects.

Conclusion

Vaniambadi S Kalyanaraman's innovative work in HIV research has led to important advancements in diagnostics and potential vaccine development. His contributions continue to impact the field significantly.