Qinghua Zhang

Title: **Qinghua Zhang: Pioneering Innovations in Zebrafish Mutant Research**

Introduction

Qinghua Zhang is a notable inventor based in Shanghai, China, known for his contributions to genetic engineering and research methodologies involving zebrafish. With a total of two patents to his name, he has made significant strides in understanding biological functions through innovative techniques.

Latest Patents

Zhang's latest patents include:

1. **Method of Preparing DDX27-Deletion Zebrafish Mutants**

This invention details a comprehensive technique for creating ddx27-deletion zebrafish mutants. It involves several steps, including determining the target for ddx27 knockout on the sixth exon, designing a gRNA sequence, and using specific primers for PCR amplification. The method culminates in the introduction of the gRNA and Cas9 protein into the zebrafish and culturing them to achieve stable inheritance of the ddx27 mutant. This innovation not only highlights the preparation method but also discloses the phenotype of the ddx27-deletion zebrafish mutant, which is crucial for investigating biological functions.

2. **Method of Constructing Zebrafish Notch1a Mutants**

This patent employs the CRISPR/Cas9 technique to construct zebrafish notch1a mutants. The method starts with identifying a target for knocking out notch1a and includes PCR amplification using specific primers. The process includes transcribing the PCR product followed by purification to obtain gRNA, which is then microinjected alongside Cas9 mRNA into zebrafish embryos. The outcome is the generation of stable notch1a mutants without affecting other genes, providing invaluable insights into the Notch signaling pathway.

Career Highlights

Qinghua Zhang's career developments are deeply rooted in his role at Shanghai Ocean University, where he contributes to advanced research and innovation in the field of marine biology and genetics. His work has garnered attention for its potential applications in biomedical research and genetic studies.

Collaborations

Throughout his career, Zhang has collaborated with esteemed colleagues such as Ce Ji and Can Shi. Their joint efforts have further enhanced the research outcomes and the scope of innovations in the area of zebrafish genetics.

Conclusion

Qinghua Zhang stands out as an influential inventor in the field of genetic engineering, particularly through his inventive approaches to creating zebrafish mutants. His contributions, reflected in his patents, mark significant advancements in biological research, paving the way for future innovations in the understanding of genetic pathways and their physiological implications.