Pauline Rudd

Title: **The Innovations of Pauline Rudd: Pioneering Glycosylation Marker Research**

Introduction

Pauline Rudd, a distinguished inventor based in Abingdon, GB, has made significant contributions to the field of biochemistry and medical diagnostics through her innovative research on glycosylation markers. With a total of two patents to her name, her work primarily focuses on identifying and quantifying physiological glycosylation markers that can play a crucial role in diagnosing and monitoring diseases, particularly cancer.

Latest Patents

Rudd's latest patents include groundbreaking techniques for the automated identification of physiological glycosylation markers. Her first patent outlines an approach that enables the identification and quantification of these markers using quantitative High-Performance Liquid Chromatography (HPLC) analysis. This method involves releasing glycans from unpurified glycoproteins, which may consist of total glycoproteins or select fractions. The glycosylation markers identified can either be native glycans or digestion products separated and amplified through exoglycosidase digestions.

Her second patent extends this innovative approach to cancer diagnostics. The process again utilizes quantitative HPLC analysis to identify and quantify glycosylation markers in glycoproteins. This methodology not only aids in diagnosing and monitoring cancer in subjects but also allows for the identification of glycoprotein biomarkers associated with these glycosylation markers, significantly enhancing cancer diagnostic capabilities. The research emphasizes the separation of glycoforms of glycoproteins on a 2D gel, providing a spectrum of biomarkers for clinical assessment.

Career Highlights

Pauline Rudd has held key positions in prominent institutions such as the National Institute for Bioprocessing Research and Training Limited (NIBRT). Her extensive research and involvement in various projects highlight her commitment to advancing our understanding of glycosylation's role in health and disease.

Collaborations

Throughout her career, Rudd has collaborated with esteemed colleagues, including Raymond A. Dwek and Catherine M. Radcliffe. These associations have enriched her research endeavors and broadened the impact of her work within the scientific community.

Conclusion

Pauline Rudd stands as a vital figure in the field of glycobiology, showcasing the potential of glycosylation markers in clinical diagnostics. Her two patents exemplify innovation in healthcare technology, with the promise of improving disease diagnosis and monitoring. Through her work, Rudd not only enhances our understanding of molecular biology but also contributes to the ongoing quest for improved medical solutions for patients worldwide.