Company Filing History:
Years Active: 2015-2017
Title: Innovations of Nicholas Rice in PCR Technology
Introduction
Nicholas Rice is an accomplished inventor based in Quincy, MA (US). He has made significant contributions to the field of polymerase chain reaction (PCR) technology. With a total of 2 patents, his work focuses on enhancing the efficiency and accuracy of DNA amplification processes.
Latest Patents
Nicholas Rice's latest patents include innovative reagents and methods for PCR. These modified double-stranded oligonucleotides possess terminal regions on each strand, with a hybrid length of 6-50 nucleotides. They have a melting temperature (Tm) of at least 32°C and include 2-4 modifying groups, each covalently attached to different terminal regions. These modifying groups are designed to be polycyclic substituents that do not have bulky non-planar portions. The modified oligonucleotides are capable of binding to the 5' exonuclease domains of DNA polymerases. When included in PCR or other primer-dependent DNA amplification reactions at concentrations generally not exceeding 2000 nM, they effectively suppress mispriming, increase polymerase selectivity against 3' terminal mismatches, and reduce scatter among replicates. Additionally, they suppress polymerase 5' exonuclease activity and inhibit polymerase activity, making them valuable for amplification reaction mixtures, assays, and kits.
Career Highlights
Nicholas Rice is affiliated with Brandeis University, where he continues to advance research in molecular biology and genetics. His work has garnered attention for its potential applications in various scientific fields, including medical diagnostics and genetic research.
Collaborations
Some of his notable coworkers include Lawrence J Wangh and John E Rice, who have contributed to his research endeavors.
Conclusion
Nicholas Rice's innovations in PCR technology demonstrate his commitment to improving DNA amplification methods. His patents reflect a deep understanding of molecular biology and a drive to enhance scientific research capabilities.