Michael Volny

Title: Michael Volny: Innovator in Surface Modification Techniques

Introduction

Michael Volny is a notable inventor based in Prague, Czech Republic. He has made significant contributions to the field of surface modification, particularly in the context of mass spectrometry and immunochemical assays. With a total of 2 patents, his work focuses on enhancing the efficiency of analyte preconcentration from complex samples.

Latest Patents

Volny's latest patents include a method of surface modification by proteins for analyte preconcentration for desorption-ionization mass spectrometry techniques and for immunochemical assays. This innovative method involves the modification of solid substrates with proteins to facilitate efficient surface preconcentration of analytes. The process utilizes electronebulization, or electrospraying, of protein solutions, which can include enzymes, lectins, or antibodies. The resulting charged electrospray is dried in real time, allowing for the formation of desolvated ions that bind firmly to the surface. This modified surface can then be used for selective interactions with affinity partners, enabling preconcentration or enzymatic modifications followed by analysis.

Another significant patent by Volny is a method of surface modification for analyzing phosphorylated peptides. This method enhances the preconcentration of phosphorylated peptides from complex mixtures prior to detection through desorption/ionization mass spectrometry. The surface functionalization is achieved through ambient ion landing, utilizing electronebulization of organometallic compounds, particularly those related to zirconium, hafnium, and titanium. The generated charged electrospray forms a stable oxide layer on the surface, which is effective for phosphopeptide enrichment.

Career Highlights

Throughout his career, Michael Volny has worked at the Institute of Microbiology AS CR, v.v.i. His research and innovations have significantly impacted the field of mass spectrometry and related applications.

Collaborations

Volny has collaborated with notable colleagues, including Petr Novak and Petr Pompach, contributing to advancements in their shared field of expertise.

Conclusion

Michael Volny's innovative methods in surface modification have paved the way for enhanced analytical techniques in mass spectrometry and immunochemical assays. His contributions continue to influence the scientific community and improve the efficiency of analyte detection.