Michael John Booth

Title: Michael John Booth: Innovator in Nucleotide Modification Detection

Introduction

Michael John Booth is a prominent inventor based in Cambridge, GB. He has made significant contributions to the field of nucleotide modification detection, holding 2 patents that focus on innovative methods for identifying modified cytosine residues.

Latest Patents

His latest patents include methods for the detection of nucleotide modification. This invention relates to the identification of modified cytosine residues, such as 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC), and 5-formylcytosine (5fC), which can be distinguished from cytosine (C) in a sample nucleotide sequence. The methods may involve oxidizing or reducing a first portion of polynucleotides that comprise the sample nucleotide sequence. Following this, the oxidized or reduced first portion and a second portion of polynucleotides are treated with bisulfite. The sequencing of the polynucleotides in both portions produces first and second nucleotide sequences, respectively. Finally, the residue in these sequences that corresponds to a cytosine residue in the sample nucleotide sequence is identified. These methods are particularly useful in the analysis of genomic DNA and RNA.

Career Highlights

Michael John Booth is associated with Cambridge Epigenetix Limited, where he applies his expertise in epigenetics and nucleotide analysis. His work has contributed to advancements in understanding genetic modifications and their implications in various biological processes.

Collaborations

He collaborates with notable figures in the field, including Shankar Balasubramanian, enhancing the impact of his research through shared knowledge and expertise.

Conclusion

Michael John Booth's innovative work in nucleotide modification detection exemplifies the importance of research in genetics and its applications in understanding complex biological systems. His contributions continue to influence the field significantly.