Margaret L Sutherland

Title: Innovations of Margaret L Sutherland

Introduction

Margaret L Sutherland is a notable inventor based in Cambridge, GB. She has made significant contributions to the field of biotechnology, particularly in the area of recombinant DNA technology. With a total of 2 patents, her work has had a substantial impact on the methods used for protein excretion in E. coli.

Latest Patents

One of her latest patents focuses on the excretion of heterologous proteins from E. coli. This recombinant DNA construct is designed to facilitate the secretion of heterologous proteins into the periplasm. It comprises a coding region where DNA coding for the heterologous protein is coupled in reading frame with DNA coding for the OmpA signal peptide. This innovative approach enables the expression of the protein in an E. coli host, utilizing a control region that includes the tac promoter, the lac operator, and a consensus ribosome binding site. The significance of this construct lies in its ability to achieve consistent excretion of various heterologous proteins without the need for carrier proteins or membrane-lytic agents, using healthy hosts instead of mutant strains with defective outer membranes.

Career Highlights

Throughout her career, Margaret has worked with several prominent companies, including Allelix, Inc. and Astra AB. Her expertise in biotechnology has allowed her to contribute to various projects that advance the field.

Collaborations

Margaret has collaborated with notable professionals, including W K Wong, enhancing her research and development efforts.

Conclusion

Margaret L Sutherland's innovative work in recombinant DNA technology has paved the way for advancements in protein excretion methods. Her contributions continue to influence the biotechnology industry and inspire future research.