Makoto Shinohara

Title: Makoto Shinohara: Innovator in Esterase Research

Introduction

Makoto Shinohara is a prominent inventor based in Aichi, Japan. He has made significant contributions to the field of biotechnology, particularly in the development of novel enzymes. His work has implications for various applications in agriculture and bio-oriented research.

Latest Patents

Shinohara holds a patent for a novel esterase derived from the sp. 0-0013 strain (FERM BP-08660). This enzyme exhibits unique properties, including the ability to hydrolyze methyl 3-hydroxypalmitate to produce 3-hydroxypalmitic acid and methanol. The optimal functioning temperature for this enzyme is 37°C, with a stable pH range between pH 7 and pH 10. Additionally, 97% of the enzyme remains stable at 43°C. It is activated by sodium and potassium ions, while being inhibited by strontium and iron ions, as well as methyl palmitate. The molecular weight of the enzyme is approximately 46,500 Da, and its isoelectric point is pI 4.

Career Highlights

Shinohara is affiliated with the National Agriculture and Bio-oriented Research Organization, where he conducts his research. His work has been instrumental in advancing the understanding of enzyme functionality and stability, contributing to the field of agricultural biotechnology.

Collaborations

He has collaborated with notable colleagues, including Yoichi Uehara and Akimasa Nakano, to further enhance the research and development of innovative biotechnological solutions.

Conclusion

Makoto Shinohara's contributions to enzyme research exemplify the importance of innovation in biotechnology. His patented esterase showcases the potential for advancements in agricultural applications.