Title: Karl Guegler - Innovator in Nanopore Sequencing Technologies.
Introduction: Karl Guegler is an inventor based in South San Francisco, CA (US). He is currently associated with Quantapore, Inc. and has made significant contributions to the field of nanopore sequencing technologies. Although he does not hold any granted patents, his work is focused on advancing methods for analyzing polynucleotides.
Latest Patent Applications: Karl Guegler has filed several innovative patent applications, including:
1. **TWO-COLOR NANOPORE SEQUENCING** - This invention is directed to methods for analyzing polymers comprising linear chains of at least two types of monomers, such as polynucleotides, including DNA and RNA. The method utilizes nanopores and optical detection to label different kinds of nucleotides with distinguishable optical signals. The labeled strands are translocated through nanopores, generating a sequence of optical signals that form an optical signature. This approach allows for the determination of complete nucleotide sequences from both sense and antisense strands of target polynucleotides.
2. **METHOD OF TRANSLOCATING NUCLEIC ACIDS THROUGH NANOPORES** - This invention provides methods for analyzing polynucleotides using nanopores that permit the passage of single-stranded polynucleotides while restricting double-stranded ones. The method involves producing a double-stranded product with a labeled strand that has a single-stranded tail. By applying an electric field across the nanopores, the single-stranded tail can be captured, allowing the labeled strand to translocate by unzipping from the double-stranded product. The ionic composition and electric field strength are carefully selected to ensure that nucleotides translocate at a controlled rate.
Conclusion: Karl Guegler is a notable inventor in the field of nanopore sequencing, contributing innovative methods that enhance the analysis of polynucleotides. His latest patent applications reflect his commitment to advancing this important area of research.