Company Filing History:
Years Active: 1991-1992
Title: Innovations of John Shine in Rhizobial Research
Introduction
John Shine is an accomplished inventor based in Sydney, Australia, known for his significant contributions to the field of rhizobial research. He holds three patents that focus on the development of diagnostic probes and promoters for rhizobium species, which play a crucial role in agricultural biotechnology.
Latest Patents
One of his latest patents involves rhizobial diagnostic probes and rhizobium trifolii nifH promoters. This invention provides useful promoters from the R. trifolii nifH gene for the construction of recombinant molecules to regulate foreign genes for expression under desired conditions. In particular, the promoters act to control the expression of foreign genes within root nodules formed by rhizobial bacterial strains in symbiotic combination with host plants. Additionally, a rhizobium diagnostic segment (RDS) is provided, comprising a DNA segment found at more than one location in rhizobia. The RDS is species-specific and detectable by DNA hybridization under stringent conditions. Methods are also provided for identifying species and strains of field isolates of Rhizobium using RDS's.
Another significant patent focuses on the nifH promoters of Bradyrhizobium japonicum and Bradyrhizobium sp. (parasponia), which have been sequenced and found to be significantly homologous. Recombinant DNA molecules comprising foreign genes under the control of such promoters are provided, which are especially useful for expressing desired foreign genes within root nodules.
Career Highlights
Throughout his career, John Shine has worked with notable companies such as Lubrizol Genetics, Inc. and The Lubrizol Corporation. His work has significantly advanced the understanding and application of rhizobial technology in agriculture.
Collaborations
John has collaborated with esteemed colleagues, including Kieran F. Scott and Barry G. Rolfe, contributing to the advancement of research in his field.
Conclusion
John Shine's innovative work in rhizobial research has led to valuable patents that enhance agricultural practices through biotechnology. His contributions continue to impact the field positively.