John N Saddler

Title: The Innovations of John N Saddler

Introduction

John N Saddler is a notable inventor based in Ottawa, Canada. He has made significant contributions to the field of biotechnology, particularly in the purification of enzymes. His work has implications for various industrial applications, especially in the processing of plant materials.

Latest Patents

One of John N Saddler's key patents is titled "Method for purifying xylanase." This patent describes a process for separating xylanases from mixtures with other hemicellulases, particularly cellulase. The method involves culturing hemicellulolytic microorganisms, specifically the fungus Trichoderma harzianum E58 and Trichoderma reesei. The separation is achieved through ultrafiltration using a membrane with a low molecular weight cut-off point, resulting in a cellulase-rich retentate and a xylanase-rich ultrafiltrate. The xylanase-rich filtrate is then concentrated and purified using adsorption and elution techniques. This innovative approach allows for the selective hydrolysis of hemicellulose, making it particularly valuable in various biotechnological applications.

Career Highlights

Throughout his career, John N Saddler has focused on advancing enzyme technology. His research has led to the development of methods that enhance the efficiency of enzyme purification. With a total of 1 patent, he has established himself as a key figure in his field.

Collaborations

John N Saddler has collaborated with notable colleagues, including Larry U Tan and Ernest K Yu. These partnerships have contributed to the advancement of his research and the successful application of his patented methods.

Conclusion

John N Saddler's innovative work in enzyme purification showcases his dedication to advancing biotechnology. His contributions have the potential to impact various industries, particularly in the efficient processing of plant materials.