Jo Caine

Title: Jo Caine: Innovator in Protein Characterization

Introduction

Jo Caine is a notable inventor based in Parkville, Australia. He has made significant contributions to the field of protein characterization, particularly through his innovative methods that enhance the understanding of protein stability and quantitation.

Latest Patents

Jo Caine holds a patent for "LC-MS/MS-based methods for characterizing proteins." This patent describes methods to analyze the in vitro stability of recalcitrant or membrane-bound proteins in simulated gastric fluid (SGF) that includes the proteolytic enzyme, pepsin. The methods utilize a novel pepsin-trypsin assay combined with advanced mass spectrometric approaches, such as LC-MS/MS, to monitor precise degradation products. The extent of protein digestion is evaluated by observing the appearance of peptic products and the disappearance of tryptic peptide products, serving as a proxy for intact protein. Additionally, the methods provide high-sensitivity LC-MRM-MS quantification for protein quantitation, which is particularly useful for characterizing proteins produced in transgenic plants, such as canola engineered to produce long-chain omega-3 polyunsaturated fatty acids.

Career Highlights

Throughout his career, Jo Caine has worked with prominent organizations, including Nuseed Nutritional Australia Pty Ltd and the Commonwealth Scientific and Industrial Research Organisation. His work has significantly advanced the field of protein analysis and has implications for nutritional science and biotechnology.

Collaborations

Jo has collaborated with esteemed colleagues, including Susan MacIntosh and Michelle Colgrave, contributing to various research projects that enhance the understanding of protein functionality and stability.

Conclusion

Jo Caine's innovative approaches to protein characterization have established him as a key figure in the field. His contributions continue to influence research and development in biotechnology and nutritional science.