Jane M Sottile

Title: The Innovative Work of Jane M. Sottile in Protein Purification

Introduction

Jane M. Sottile, an accomplished inventor located in Madison, WI, has made significant contributions to the field of protein purification. With two patents to her name, her innovative work focuses on enhancing the efficiency of recombinant protein purification through strategic molecular design.

Latest Patents

Sottile's latest patents center around a fibronectin purification vector. These vectors disclose methods and vectors for recombinant protein purification by incorporating a DNA sequence that codes for a gelatin-binding region of fibronectin. The vectors allow the expression of a foreign DNA sequence of interest linked to the fibronectin component. The inclusion of secretion signals enables the successful secretion of the product from production cells. After secretion, the product can be purified using a gelatin-containing affinity column, followed by digestion with proteases, such as trypsin, to cleave the desired protein from the gelatin-binding region. The resulting protein is left with no more than one extraneous amino acid, thus simplifying the purification process.

Career Highlights

Sottile currently works at the Wisconsin Alumni Research Foundation, where she has been able to leverage her expertise to drive advancements in protein purification technologies. Her inventive approach and dedication to research have positioned her as a prominent figure in her field, contributing to more efficient methodologies in biotechnology.

Collaborations

Throughout her career, Sottile has collaborated closely with her coworker Deane F. Mosher. This partnership has fostered an environment of shared ideas and innovations, enabling the development of groundbreaking techniques in the realm of protein purification.

Conclusion

Jane M. Sottile's contributions to the field of protein purification exemplify the impact of innovative thinking in science. With her patents emphasizing the efficacious extraction of proteins, she continues to shape the future of biotechnological processes. Her work not only enhances the scientific community but also paves the way for advancements that may benefit various industries reliant on protein-based products.